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More than fishing in the dark: PCR of a dispersed sequence produces simple but ultrasensitive Wolbachia detection

BACKGROUND: Detecting intracellular bacterial symbionts can be challenging when they persist at very low densities. Wolbachia, a widespread bacterial endosymbiont of invertebrates, is particularly challenging. Although it persists at high titers in many species, in others its densities are far below...

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Detalles Bibliográficos
Autores principales: Schneider, Daniela I, Klasson, Lisa, Lind, Anders E, Miller, Wolfgang J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4029913/
https://www.ncbi.nlm.nih.gov/pubmed/24885505
http://dx.doi.org/10.1186/1471-2180-14-121