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A novel approach to the generation of seamless constructs for plant transformation
BACKGROUND: When creating plant transformation vectors, full control of nucleotides flanking the insert in the final construct may be desirable. Modern ligase-independent methods for DNA-recombination are based on linearization by classical type II restriction endonucleases (REs) alone or in combina...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030040/ https://www.ncbi.nlm.nih.gov/pubmed/24855486 http://dx.doi.org/10.1186/1746-4811-10-10 |