Cargando…

A novel approach to the generation of seamless constructs for plant transformation

BACKGROUND: When creating plant transformation vectors, full control of nucleotides flanking the insert in the final construct may be desirable. Modern ligase-independent methods for DNA-recombination are based on linearization by classical type II restriction endonucleases (REs) alone or in combina...

Descripción completa

Detalles Bibliográficos
Autores principales: Kronbak, Remy, Ingvardsen, Christina Rønn, Madsen, Claus Krogh, Gregersen, Per Langkjær
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030040/
https://www.ncbi.nlm.nih.gov/pubmed/24855486
http://dx.doi.org/10.1186/1746-4811-10-10