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A novel approach to the generation of seamless constructs for plant transformation

BACKGROUND: When creating plant transformation vectors, full control of nucleotides flanking the insert in the final construct may be desirable. Modern ligase-independent methods for DNA-recombination are based on linearization by classical type II restriction endonucleases (REs) alone or in combina...

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Detalles Bibliográficos
Autores principales:	Kronbak, Remy, Ingvardsen, Christina Rønn, Madsen, Claus Krogh, Gregersen, Per Langkjær
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030040/ https://www.ncbi.nlm.nih.gov/pubmed/24855486 http://dx.doi.org/10.1186/1746-4811-10-10

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