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Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9

Toxoplasma gondii has become a model for studying the phylum Apicomplexa, in part due to the availability of excellent genetic tools. Although reverse genetic tools are available in a few widely utilized laboratory strains, they rely on special genetic backgrounds that are not easily implemented in...

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Detalles Bibliográficos
Autores principales: Shen, Bang, Brown, Kevin M., Lee, Tobie D., Sibley, L. David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030483/
https://www.ncbi.nlm.nih.gov/pubmed/24825012
http://dx.doi.org/10.1128/mBio.01114-14