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Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9
Toxoplasma gondii has become a model for studying the phylum Apicomplexa, in part due to the availability of excellent genetic tools. Although reverse genetic tools are available in a few widely utilized laboratory strains, they rely on special genetic backgrounds that are not easily implemented in...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4030483/ https://www.ncbi.nlm.nih.gov/pubmed/24825012 http://dx.doi.org/10.1128/mBio.01114-14 |