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Transposon-mediated targeted and specific knockdown of maternally expressed transcripts in the ascidian Ciona intestinalis

Maternal mRNAs play crucial roles during early embryogenesis of ascidians, but their functions are largely unknown. In this study, we developed a new method to specifically knockdown maternal mRNAs in Ciona intestinalis using transposon-mediated transgenesis. We found that GFP expression is epigenet...

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Detalles Bibliográficos
Autores principales: Iitsuka, Takako, Mita, Kaoru, Hozumi, Akiko, Hamada, Mayuko, Satoh, Nori, Sasakura, Yasunori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4031475/
https://www.ncbi.nlm.nih.gov/pubmed/24854849
http://dx.doi.org/10.1038/srep05050