Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

RNA-guided endonucleases (RGENs) derived from the CRISPR/Cas system represent an efficient tool for genome editing. RGENs consist of two components: Cas9 protein and guide RNA. Plasmid-mediated delivery of these components into cells can result in uncontrolled integration of the plasmid sequence int...

Descripción completa

Detalles Bibliográficos
Autores principales: Ramakrishna, Suresh, Kwaku Dad, Abu-Bonsrah, Beloor, Jagadish, Gopalappa, Ramu, Lee, Sang-Kyung, Kim, Hyongbum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032848/
https://www.ncbi.nlm.nih.gov/pubmed/24696462
http://dx.doi.org/10.1101/gr.171264.113

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032848/
https://www.ncbi.nlm.nih.gov/pubmed/24696462
http://dx.doi.org/10.1101/gr.171264.113

Ejemplares similares