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Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA
RNA-guided endonucleases (RGENs) derived from the CRISPR/Cas system represent an efficient tool for genome editing. RGENs consist of two components: Cas9 protein and guide RNA. Plasmid-mediated delivery of these components into cells can result in uncontrolled integration of the plasmid sequence int...
Autores principales: | Ramakrishna, Suresh, Kwaku Dad, Abu-Bonsrah, Beloor, Jagadish, Gopalappa, Ramu, Lee, Sang-Kyung, Kim, Hyongbum |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032848/ https://www.ncbi.nlm.nih.gov/pubmed/24696462 http://dx.doi.org/10.1101/gr.171264.113 |
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