The pyrE Gene as a Bidirectional Selection Marker in Bifidobacterium Longum 105-A

We constructed a deletion mutant of the pyrE gene in Bifidobacterium longum 105-A. A pyrE knockout cassette was cloned into pKKT427, a Bifidobacterium-Escherichia coli shuttle vector, and then introduced into B. longum 105-A by electroporation. The transformants were propagated and spread onto MRS p...

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Detalles Bibliográficos
Autores principales: SAKAGUCHI, Kouta, FUNAOKA, Nobutaka, TANI, Saori, HOBO, Aya, MITSUNAGA, Tohru, KANO, Yasunobu, SUZUKI, Tohru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscience of Microbiota, Food and Health 2013
Materias:
Full Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034322/
https://www.ncbi.nlm.nih.gov/pubmed/24936363
http://dx.doi.org/10.12938/bmfh.32.59

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034322/
https://www.ncbi.nlm.nih.gov/pubmed/24936363
http://dx.doi.org/10.12938/bmfh.32.59

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