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The pyrE Gene as a Bidirectional Selection Marker in Bifidobacterium Longum 105-A
We constructed a deletion mutant of the pyrE gene in Bifidobacterium longum 105-A. A pyrE knockout cassette was cloned into pKKT427, a Bifidobacterium-Escherichia coli shuttle vector, and then introduced into B. longum 105-A by electroporation. The transformants were propagated and spread onto MRS p...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Bioscience of Microbiota, Food and Health
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034322/ https://www.ncbi.nlm.nih.gov/pubmed/24936363 http://dx.doi.org/10.12938/bmfh.32.59 |