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Follicular Viability and Histological Alterations after Auto-transplantation of Dog Ovaries by Experimentally Inducing Blood Sinus on Stomach

BACKGROUND: Currently, chemotherapy and radiotherapy are considered most effective methods for cancer treatment, however these strategies often result in fertility problems. A favorable alternative to prevent fertility loss in cancer patients is the cryopreservation and transplantation of sexual tis...

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Autores principales: Khoram, Hazhir, Najafpour, Alireza, Razi, Mazdak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040240/
https://www.ncbi.nlm.nih.gov/pubmed/24917921
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author Khoram, Hazhir
Najafpour, Alireza
Razi, Mazdak
author_facet Khoram, Hazhir
Najafpour, Alireza
Razi, Mazdak
author_sort Khoram, Hazhir
collection PubMed
description BACKGROUND: Currently, chemotherapy and radiotherapy are considered most effective methods for cancer treatment, however these strategies often result in fertility problems. A favorable alternative to prevent fertility loss in cancer patients is the cryopreservation and transplantation of sexual tissues (ovaries and/or testes). There is a low rate of fertilization following cryopreservation of ovaries prior to implantation. Therefore, in our opinion, this low rate is caused by instable blood flow during organ transplantation. Thus, this study researches a canine ovarian model that focuses on direct exposure of ovaries with blood in an experimentally induced sinus-like cavity. We implanted this tissue on the muscular layer of the stomach, which is its most vascularized region. MATERIALS AND METHODS: Ovarian transplantation was conducted on T1 animals (n=5), bilateral ovariectomy was performed on T2 animals (n=5), unilateral ovariectomy was conducted on T3 cases and animals in the control-sham group (n=5) did not undergo ovariectomy or transplantation. RESULTS: All isotransplanted ovaries survived. Ovaries resumed follicular growth and revascularization. Transplanted ovaries contained 75%-76% of survived small follicles (pre antral) after 60 days. The ovarian granulosa cells showed considerable resistance against ischemia. After day 30 no statistically significant differences in the level of estradiol and progesterone were observed between T1 animals and the T3 group. T1 animals showed considerably high levels of progesterone and estradiol in comparison to T2 cases. CONCLUSION: This study showed that using blood sinus method for ovarian isotransplantation helps ovarian tissue to survive from post implantation ischemia which confirms with normal follicles presentation and intact endocrine function of the implanted ovaries.
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spelling pubmed-40402402014-06-10 Follicular Viability and Histological Alterations after Auto-transplantation of Dog Ovaries by Experimentally Inducing Blood Sinus on Stomach Khoram, Hazhir Najafpour, Alireza Razi, Mazdak Int J Fertil Steril Original Article BACKGROUND: Currently, chemotherapy and radiotherapy are considered most effective methods for cancer treatment, however these strategies often result in fertility problems. A favorable alternative to prevent fertility loss in cancer patients is the cryopreservation and transplantation of sexual tissues (ovaries and/or testes). There is a low rate of fertilization following cryopreservation of ovaries prior to implantation. Therefore, in our opinion, this low rate is caused by instable blood flow during organ transplantation. Thus, this study researches a canine ovarian model that focuses on direct exposure of ovaries with blood in an experimentally induced sinus-like cavity. We implanted this tissue on the muscular layer of the stomach, which is its most vascularized region. MATERIALS AND METHODS: Ovarian transplantation was conducted on T1 animals (n=5), bilateral ovariectomy was performed on T2 animals (n=5), unilateral ovariectomy was conducted on T3 cases and animals in the control-sham group (n=5) did not undergo ovariectomy or transplantation. RESULTS: All isotransplanted ovaries survived. Ovaries resumed follicular growth and revascularization. Transplanted ovaries contained 75%-76% of survived small follicles (pre antral) after 60 days. The ovarian granulosa cells showed considerable resistance against ischemia. After day 30 no statistically significant differences in the level of estradiol and progesterone were observed between T1 animals and the T3 group. T1 animals showed considerably high levels of progesterone and estradiol in comparison to T2 cases. CONCLUSION: This study showed that using blood sinus method for ovarian isotransplantation helps ovarian tissue to survive from post implantation ischemia which confirms with normal follicles presentation and intact endocrine function of the implanted ovaries. Royan Institute 2011 2011-03-21 /pmc/articles/PMC4040240/ /pubmed/24917921 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Khoram, Hazhir
Najafpour, Alireza
Razi, Mazdak
Follicular Viability and Histological Alterations after Auto-transplantation of Dog Ovaries by Experimentally Inducing Blood Sinus on Stomach
title Follicular Viability and Histological Alterations after Auto-transplantation of Dog Ovaries by Experimentally Inducing Blood Sinus on Stomach
title_full Follicular Viability and Histological Alterations after Auto-transplantation of Dog Ovaries by Experimentally Inducing Blood Sinus on Stomach
title_fullStr Follicular Viability and Histological Alterations after Auto-transplantation of Dog Ovaries by Experimentally Inducing Blood Sinus on Stomach
title_full_unstemmed Follicular Viability and Histological Alterations after Auto-transplantation of Dog Ovaries by Experimentally Inducing Blood Sinus on Stomach
title_short Follicular Viability and Histological Alterations after Auto-transplantation of Dog Ovaries by Experimentally Inducing Blood Sinus on Stomach
title_sort follicular viability and histological alterations after auto-transplantation of dog ovaries by experimentally inducing blood sinus on stomach
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040240/
https://www.ncbi.nlm.nih.gov/pubmed/24917921
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