Progressive engineering of a homing endonuclease genome editing reagent for the murine X-linked immunodeficiency locus

LAGLIDADG homing endonucleases (LHEs) are compact endonucleases with 20–22 bp recognition sites, and thus are ideal scaffolds for engineering site-specific DNA cleavage enzymes for genome editing applications. Here, we describe a general approach to LHE engineering that combines rational design with...

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Detalles Bibliográficos
Autores principales: Wang, Yupeng, Khan, Iram F., Boissel, Sandrine, Jarjour, Jordan, Pangallo, Joseph, Thyme, Summer, Baker, David, Scharenberg, Andrew M., Rawlings, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041414/
https://www.ncbi.nlm.nih.gov/pubmed/24682825
http://dx.doi.org/10.1093/nar/gku224

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041414/
https://www.ncbi.nlm.nih.gov/pubmed/24682825
http://dx.doi.org/10.1093/nar/gku224

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