iRFP Is a Real Time Marker for Transformation Based Assays in High Content Screening

Anchorage independent growth is one of the hallmarks of oncogenic transformation. Here we show that infrared fluorescent protein (iRFP) based assays allow accurate and unbiased determination of colony formation and anchorage independent growth over time. This protocol is particularly compatible with...

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Detalles Bibliográficos
Autores principales: Paulus-Hock, Viola, Cheung, Eric C., Roxburgh, Patricia, Vousden, Karen H., Hock, Andreas K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041769/
https://www.ncbi.nlm.nih.gov/pubmed/24887316
http://dx.doi.org/10.1371/journal.pone.0098399

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041769/
https://www.ncbi.nlm.nih.gov/pubmed/24887316
http://dx.doi.org/10.1371/journal.pone.0098399

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