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iRFP Is a Real Time Marker for Transformation Based Assays in High Content Screening
Anchorage independent growth is one of the hallmarks of oncogenic transformation. Here we show that infrared fluorescent protein (iRFP) based assays allow accurate and unbiased determination of colony formation and anchorage independent growth over time. This protocol is particularly compatible with...
Autores principales: | Paulus-Hock, Viola, Cheung, Eric C., Roxburgh, Patricia, Vousden, Karen H., Hock, Andreas K. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041769/ https://www.ncbi.nlm.nih.gov/pubmed/24887316 http://dx.doi.org/10.1371/journal.pone.0098399 |
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