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Directed Evolution of Human Heavy Chain Variable Domain (V(H)) Using In Vivo Protein Fitness Filter
Human immunoglobulin heavy chain variable domains (V(H)) are promising scaffolds for antigen binding. However, V(H) is an unstable and aggregation-prone protein, hindering its use for therapeutic purposes. To evolve the V(H) domain, we performed in vivo protein solubility selection that linked antib...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043505/ https://www.ncbi.nlm.nih.gov/pubmed/24892548 http://dx.doi.org/10.1371/journal.pone.0098178 |