Directed Evolution of Human Heavy Chain Variable Domain (V(H)) Using In Vivo Protein Fitness Filter

Human immunoglobulin heavy chain variable domains (V(H)) are promising scaffolds for antigen binding. However, V(H) is an unstable and aggregation-prone protein, hindering its use for therapeutic purposes. To evolve the V(H) domain, we performed in vivo protein solubility selection that linked antib...

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Detalles Bibliográficos
Autores principales: Kim, Dong-Sik, Song, Hyung-Nam, Nam, Hyo Jung, Kim, Sung-Geun, Park, Young-Seoub, Park, Jae-Chan, Woo, Eui-Jeon, Lim, Hyung-Kwon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043505/
https://www.ncbi.nlm.nih.gov/pubmed/24892548
http://dx.doi.org/10.1371/journal.pone.0098178

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