Cargando…

New insight into the SSC8 genetic determination of fatty acid composition in pigs

BACKGROUND: Fat content and fatty acid composition in swine are becoming increasingly studied because of their effect on sensory and nutritional quality of meat. A QTL (quantitative trait locus) for fatty acid composition in backfat was previously detected on porcine chromosome 8 (SSC8) in an Iberia...

Descripción completa

Detalles Bibliográficos
Autores principales: Revilla, Manuel, Ramayo-Caldas, Yuliaxis, Castelló, Anna, Corominas, Jordi, Puig-Oliveras, Anna, Ibáñez-Escriche, Noelia, Muñoz, María, Ballester, Maria, Folch, Josep M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043687/
https://www.ncbi.nlm.nih.gov/pubmed/24758572
http://dx.doi.org/10.1186/1297-9686-46-28
_version_ 1782318964933656576
author Revilla, Manuel
Ramayo-Caldas, Yuliaxis
Castelló, Anna
Corominas, Jordi
Puig-Oliveras, Anna
Ibáñez-Escriche, Noelia
Muñoz, María
Ballester, Maria
Folch, Josep M
author_facet Revilla, Manuel
Ramayo-Caldas, Yuliaxis
Castelló, Anna
Corominas, Jordi
Puig-Oliveras, Anna
Ibáñez-Escriche, Noelia
Muñoz, María
Ballester, Maria
Folch, Josep M
author_sort Revilla, Manuel
collection PubMed
description BACKGROUND: Fat content and fatty acid composition in swine are becoming increasingly studied because of their effect on sensory and nutritional quality of meat. A QTL (quantitative trait locus) for fatty acid composition in backfat was previously detected on porcine chromosome 8 (SSC8) in an Iberian x Landrace F(2) intercross. More recently, a genome-wide association study detected the same genomic region for muscle fatty acid composition in an Iberian x Landrace backcross population. ELOVL6, a strong positional candidate gene for this QTL, contains a polymorphism in its promoter region (ELOVL6:c.-533C < T), which is associated with percentage of palmitic and palmitoleic acids in muscle and adipose tissues. Here, a combination of single-marker association and the haplotype-based approach was used to analyze backfat fatty acid composition in 470 animals of an Iberian x Landrace F(2) intercross genotyped with 144 SNPs (single nucleotide polymorphisms) distributed along SSC8. RESULTS: Two trait-associated SNP regions were identified at 93 Mb and 119 Mb on SSC8. The strongest statistical signals of both regions were observed for palmitoleic acid (C16:1(n-7)) content and C18:0/C16:0 and C18:1(n-7)/C16:1(n-7) elongation ratios. MAML3 and SETD7 are positional candidate genes in the 93 Mb region and two novel microsatellites in MAML3 and nine SNPs in SETD7 were identified. No significant association for the MAML3 microsatellite genotypes was detected. The SETD7:c.700G > T SNP, although statistically significant, was not the strongest signal in this region. In addition, the expression of MAML3 and SETD7 in liver and adipose tissue varied among animals, but no association was detected with the polymorphisms in these genes. In the 119 Mb region, the ELOVL6:c.-533C > T polymorphism showed a strong association with percentage of palmitic and palmitoleic fatty acids and elongation ratios in backfat. CONCLUSIONS: Our results suggest that the polymorphisms studied in MAML3 and SETD7 are not the causal mutations for the QTL in the 93 Mb region. However, the results for ELOVL6 support the hypothesis that the ELOVL6:c.-533C > T polymorphism has a pleiotropic effect on backfat and intramuscular fatty acid composition and that it has a role in the determination of the QTL in the 119 Mb region.
format Online
Article
Text
id pubmed-4043687
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-40436872014-06-17 New insight into the SSC8 genetic determination of fatty acid composition in pigs Revilla, Manuel Ramayo-Caldas, Yuliaxis Castelló, Anna Corominas, Jordi Puig-Oliveras, Anna Ibáñez-Escriche, Noelia Muñoz, María Ballester, Maria Folch, Josep M Genet Sel Evol Research BACKGROUND: Fat content and fatty acid composition in swine are becoming increasingly studied because of their effect on sensory and nutritional quality of meat. A QTL (quantitative trait locus) for fatty acid composition in backfat was previously detected on porcine chromosome 8 (SSC8) in an Iberian x Landrace F(2) intercross. More recently, a genome-wide association study detected the same genomic region for muscle fatty acid composition in an Iberian x Landrace backcross population. ELOVL6, a strong positional candidate gene for this QTL, contains a polymorphism in its promoter region (ELOVL6:c.-533C < T), which is associated with percentage of palmitic and palmitoleic acids in muscle and adipose tissues. Here, a combination of single-marker association and the haplotype-based approach was used to analyze backfat fatty acid composition in 470 animals of an Iberian x Landrace F(2) intercross genotyped with 144 SNPs (single nucleotide polymorphisms) distributed along SSC8. RESULTS: Two trait-associated SNP regions were identified at 93 Mb and 119 Mb on SSC8. The strongest statistical signals of both regions were observed for palmitoleic acid (C16:1(n-7)) content and C18:0/C16:0 and C18:1(n-7)/C16:1(n-7) elongation ratios. MAML3 and SETD7 are positional candidate genes in the 93 Mb region and two novel microsatellites in MAML3 and nine SNPs in SETD7 were identified. No significant association for the MAML3 microsatellite genotypes was detected. The SETD7:c.700G > T SNP, although statistically significant, was not the strongest signal in this region. In addition, the expression of MAML3 and SETD7 in liver and adipose tissue varied among animals, but no association was detected with the polymorphisms in these genes. In the 119 Mb region, the ELOVL6:c.-533C > T polymorphism showed a strong association with percentage of palmitic and palmitoleic fatty acids and elongation ratios in backfat. CONCLUSIONS: Our results suggest that the polymorphisms studied in MAML3 and SETD7 are not the causal mutations for the QTL in the 93 Mb region. However, the results for ELOVL6 support the hypothesis that the ELOVL6:c.-533C > T polymorphism has a pleiotropic effect on backfat and intramuscular fatty acid composition and that it has a role in the determination of the QTL in the 119 Mb region. BioMed Central 2014-04-23 /pmc/articles/PMC4043687/ /pubmed/24758572 http://dx.doi.org/10.1186/1297-9686-46-28 Text en Copyright © 2014 Revilla et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Revilla, Manuel
Ramayo-Caldas, Yuliaxis
Castelló, Anna
Corominas, Jordi
Puig-Oliveras, Anna
Ibáñez-Escriche, Noelia
Muñoz, María
Ballester, Maria
Folch, Josep M
New insight into the SSC8 genetic determination of fatty acid composition in pigs
title New insight into the SSC8 genetic determination of fatty acid composition in pigs
title_full New insight into the SSC8 genetic determination of fatty acid composition in pigs
title_fullStr New insight into the SSC8 genetic determination of fatty acid composition in pigs
title_full_unstemmed New insight into the SSC8 genetic determination of fatty acid composition in pigs
title_short New insight into the SSC8 genetic determination of fatty acid composition in pigs
title_sort new insight into the ssc8 genetic determination of fatty acid composition in pigs
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043687/
https://www.ncbi.nlm.nih.gov/pubmed/24758572
http://dx.doi.org/10.1186/1297-9686-46-28
work_keys_str_mv AT revillamanuel newinsightintothessc8geneticdeterminationoffattyacidcompositioninpigs
AT ramayocaldasyuliaxis newinsightintothessc8geneticdeterminationoffattyacidcompositioninpigs
AT castelloanna newinsightintothessc8geneticdeterminationoffattyacidcompositioninpigs
AT corominasjordi newinsightintothessc8geneticdeterminationoffattyacidcompositioninpigs
AT puigoliverasanna newinsightintothessc8geneticdeterminationoffattyacidcompositioninpigs
AT ibanezescrichenoelia newinsightintothessc8geneticdeterminationoffattyacidcompositioninpigs
AT munozmaria newinsightintothessc8geneticdeterminationoffattyacidcompositioninpigs
AT ballestermaria newinsightintothessc8geneticdeterminationoffattyacidcompositioninpigs
AT folchjosepm newinsightintothessc8geneticdeterminationoffattyacidcompositioninpigs