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Enhanced Specialized Transduction Using Recombineering in Mycobacterium tuberculosis

Genetic engineering has contributed greatly to our understanding of Mycobacterium tuberculosis biology and has facilitated antimycobacterial and vaccine development. However, methods to generate M. tuberculosis deletion mutants remain labor-intensive and relatively inefficient. Here, methods are des...

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Detalles Bibliográficos
Autores principales: Tufariello, JoAnn M., Malek, Adel A., Vilchèze, Catherine, Cole, Laura E., Ratner, Hannah K., González, Pablo A., Jain, Paras, Hatfull, Graham F., Larsen, Michelle H., Jacobs, William R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Microbiology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045075/
https://www.ncbi.nlm.nih.gov/pubmed/24865558
http://dx.doi.org/10.1128/mBio.01179-14