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Enhanced Specialized Transduction Using Recombineering in Mycobacterium tuberculosis
Genetic engineering has contributed greatly to our understanding of Mycobacterium tuberculosis biology and has facilitated antimycobacterial and vaccine development. However, methods to generate M. tuberculosis deletion mutants remain labor-intensive and relatively inefficient. Here, methods are des...
Autores principales: | Tufariello, JoAnn M., Malek, Adel A., Vilchèze, Catherine, Cole, Laura E., Ratner, Hannah K., González, Pablo A., Jain, Paras, Hatfull, Graham F., Larsen, Michelle H., Jacobs, William R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045075/ https://www.ncbi.nlm.nih.gov/pubmed/24865558 http://dx.doi.org/10.1128/mBio.01179-14 |
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