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Structural Characterization and Function Determination of a Nonspecific Carboxylate Esterase from the Amidohydrolase Superfamily with a Promiscuous Ability To Hydrolyze Methylphosphonate Esters

[Image: see text] The uncharacterized protein Rsp3690 from Rhodobacter sphaeroides is a member of the amidohydrolase superfamily of enzymes. In this investigation the gene for Rsp3690 was expressed in Escherichia coli and purified to homogeneity, and the three-dimensional structure was determined to...

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Detalles Bibliográficos
Autores principales: Xiang, Dao Feng, Kumaran, Desigan, Swaminathan, Subramanyam, Raushel, Frank M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4045322/
https://www.ncbi.nlm.nih.gov/pubmed/24832101
http://dx.doi.org/10.1021/bi5004266