Visualization of specific DNA sequences in living mouse embryonic stem cells with a programmable fluorescent CRISPR/Cas system

Labeling and tracing of specific sequences in living cells has been a major challenge in studying the spatiotemporal dynamics of native chromatin. Here we repurposed the prokaryotic CRISPR/Cas adaptive immunity system to specifically detect endogenous genomic loci in mouse embryonic stem cells. We c...

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Detalles Bibliográficos
Autores principales: Anton, Tobias, Bultmann, Sebastian, Leonhardt, Heinrich, Markaki, Yolanda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2014
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049922/
https://www.ncbi.nlm.nih.gov/pubmed/24637835
http://dx.doi.org/10.4161/nucl.28488

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049922/
https://www.ncbi.nlm.nih.gov/pubmed/24637835
http://dx.doi.org/10.4161/nucl.28488

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