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A Novel Strategy for Enrichment and Isolation of Osteoprogenitor Cells from Induced Pluripotent Stem Cells Based on Surface Marker Combination

In this study, we developed a new method to stimulate osteogenic differentiation in tissue-nonspecific alkaline phosphatase (TNAP)-positive cells liberated from human induced pluripotent stem cells (hiPSCs)-derived embryoid bodies (EBs) with 14 days long TGF-β/IGF-1/FGF-2 treatment. TNAP is a marker...

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Detalles Bibliográficos
Autores principales: Ochiai-Shino, Hiromi, Kato, Hiroshi, Sawada, Takashi, Onodera, Shoko, Saito, Akiko, Takato, Tsuyoshi, Shibahara, Takahiko, Muramatsu, Takashi, Azuma, Toshifumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050034/
https://www.ncbi.nlm.nih.gov/pubmed/24911063
http://dx.doi.org/10.1371/journal.pone.0099534