Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow

Immortal mast cell lines, such as RBL-2H3 and HMC-1 cells, are commonly utilized to investigate the function of mast cells. However, they are tumor cells carrying a gain-of-function mutation of Kit. We established an immortal mast cell line without Kit mutation, NCL-2, derived from NC mouse bone mar...

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Detalles Bibliográficos
Autores principales: Hiragun, Takaaki, Yanase, Yuhki, Okabe, Tsutomu, Hiragun, Makiko, Kawai, Mikio, Hide, Michihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050185/
https://www.ncbi.nlm.nih.gov/pubmed/24918047
http://dx.doi.org/10.1016/j.fob.2014.03.011
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author Hiragun, Takaaki
Yanase, Yuhki
Okabe, Tsutomu
Hiragun, Makiko
Kawai, Mikio
Hide, Michihiro
author_facet Hiragun, Takaaki
Yanase, Yuhki
Okabe, Tsutomu
Hiragun, Makiko
Kawai, Mikio
Hide, Michihiro
author_sort Hiragun, Takaaki
collection PubMed
description Immortal mast cell lines, such as RBL-2H3 and HMC-1 cells, are commonly utilized to investigate the function of mast cells. However, they are tumor cells carrying a gain-of-function mutation of Kit. We established an immortal mast cell line without Kit mutation, NCL-2, derived from NC mouse bone marrow. NCL-2 cells could be maintained without additional growth factors and thus could respond to exogenous growth signals. Moreover, NCL-2 cells expressed FcεRI and KIT, and release histamine and LTB(4) in response to antigen stimulation. This cell line could be a useful tool to analyze proliferation, differentiation, and function of normal mast cells.
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spelling pubmed-40501852014-06-10 Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow Hiragun, Takaaki Yanase, Yuhki Okabe, Tsutomu Hiragun, Makiko Kawai, Mikio Hide, Michihiro FEBS Open Bio Article Immortal mast cell lines, such as RBL-2H3 and HMC-1 cells, are commonly utilized to investigate the function of mast cells. However, they are tumor cells carrying a gain-of-function mutation of Kit. We established an immortal mast cell line without Kit mutation, NCL-2, derived from NC mouse bone marrow. NCL-2 cells could be maintained without additional growth factors and thus could respond to exogenous growth signals. Moreover, NCL-2 cells expressed FcεRI and KIT, and release histamine and LTB(4) in response to antigen stimulation. This cell line could be a useful tool to analyze proliferation, differentiation, and function of normal mast cells. Elsevier 2014-04-02 /pmc/articles/PMC4050185/ /pubmed/24918047 http://dx.doi.org/10.1016/j.fob.2014.03.011 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Hiragun, Takaaki
Yanase, Yuhki
Okabe, Tsutomu
Hiragun, Makiko
Kawai, Mikio
Hide, Michihiro
Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow
title Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow
title_full Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow
title_fullStr Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow
title_full_unstemmed Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow
title_short Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow
title_sort establishment of a mast cell line, ncl-2, without kit mutation, derived from nc mouse bone marrow
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4050185/
https://www.ncbi.nlm.nih.gov/pubmed/24918047
http://dx.doi.org/10.1016/j.fob.2014.03.011
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