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Excision of viral reprogramming cassettes by Cre protein transduction enables rapid, robust and efficient derivation of transgene-free human induced pluripotent stem cells
Integrating viruses represent robust tools for cellular reprogramming; however, the presence of viral transgenes in induced pluripotent stem cells (iPSCs) is deleterious because it holds the risk of insertional mutagenesis leading to malignant transformation. Here, we combine the robustness of lenti...
Autores principales: | Kadari, Asifiqbal, Lu, Min, Li, Ming, Sekaran, Thileepan, Thummer, Rajkumar P, Guyette, Naomi, Chu, Vi, Edenhofer, Frank |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055111/ https://www.ncbi.nlm.nih.gov/pubmed/24713299 http://dx.doi.org/10.1186/scrt435 |
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