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The Bias Associated with Amplicon Sequencing Does Not Affect the Quantitative Assessment of Bacterial Community Dynamics

The performance of two sets of primers targeting variable regions of the 16S rRNA gene V1–V3 and V4 was compared in their ability to describe changes of bacterial diversity and temporal turnover in full-scale activated sludge. Duplicate sets of high-throughput amplicon sequencing data of the two 16S...

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Detalles Bibliográficos
Autores principales: Ibarbalz, Federico M., Pérez, María Victoria, Figuerola, Eva L. M., Erijman, Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4055690/
https://www.ncbi.nlm.nih.gov/pubmed/24923665
http://dx.doi.org/10.1371/journal.pone.0099722