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TECHNICAL BRIEF: Optimized pipeline for isolation of high-quality RNA from corneal cell subpopulations

Purpose: Attempts to determine the transcriptional profile of discrete subsets of limbal epithelial cells in situ using laser capture microdissection (LCM) face two major challenges. First, the transcriptional profile of cells within a tissue may rapidly change as the tissue is excised and exposed t...

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Detalles Bibliográficos
Autores principales: Bath, Chris, Fink, Trine, Vorum, Henrik, Hjortdal, Jesper, Zachar, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057246/
https://www.ncbi.nlm.nih.gov/pubmed/24940035