Access of Hydrogen-Radicals to the Peptide-Backbone as a Measure for Estimating the Flexibility of Proteins Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

A factor for estimating the flexibility of proteins is described that uses a cleavage method of “in-source decay (ISD)” coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The MALDI-ISD spectra of bovine serum albumin (BSA), myoglobin and thioredoxin show discontin...

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Detalles Bibliográficos
Autores principales: Takayama, Mitsuo, Nagoshi, Keishiro, Iimuro, Ryunosuke, Inatomi, Kazuma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057740/
https://www.ncbi.nlm.nih.gov/pubmed/24828203
http://dx.doi.org/10.3390/ijms15058428

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057740/
https://www.ncbi.nlm.nih.gov/pubmed/24828203
http://dx.doi.org/10.3390/ijms15058428

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