Random Single Amino Acid Deletion Sampling Unveils Structural Tolerance and the Benefits of Helical Registry Shift on GFP Folding and Structure

Altering a protein’s backbone through amino acid deletion is a common evolutionary mutational mechanism, but is generally ignored during protein engineering primarily because its effect on the folding-structure-function relationship is difficult to predict. Using directed evolution, enhanced green f...

Descripción completa

Detalles Bibliográficos
Autores principales: Arpino, James A.J., Reddington, Samuel C., Halliwell, Lisa M., Rizkallah, Pierre J., Jones, D. Dafydd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058518/
https://www.ncbi.nlm.nih.gov/pubmed/24856363
http://dx.doi.org/10.1016/j.str.2014.03.014

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4058518/
https://www.ncbi.nlm.nih.gov/pubmed/24856363
http://dx.doi.org/10.1016/j.str.2014.03.014

Ejemplares similares