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Chemical reactivation of quenched fluorescent protein molecules enables resin-embedded fluorescence microimaging
Resin embedding is a well-established technique to prepare biological specimens for microscopic imaging. However, it is not compatible with modern green-fluorescent protein (GFP) fluorescent-labelling technique because it significantly quenches the fluorescence of GFP and its variants. Previous empi...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059927/ https://www.ncbi.nlm.nih.gov/pubmed/24886825 http://dx.doi.org/10.1038/ncomms4992 |