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Expression of Multiple Transgenes from a Single Construct Using Viral 2A Peptides in Drosophila

Expression of multiple reporter or effector transgenes in the same cell from a single construct is increasingly necessary in various experimental paradigms. The discovery of short, virus-derived peptide sequences that mediate a ribosome-skipping event enables generation of multiple separate peptide...

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Detalles Bibliográficos
Autores principales: Daniels, Richard W., Rossano, Adam J., Macleod, Gregory T., Ganetzky, Barry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063965/
https://www.ncbi.nlm.nih.gov/pubmed/24945148
http://dx.doi.org/10.1371/journal.pone.0100637