Cargando…

Expression of Multiple Transgenes from a Single Construct Using Viral 2A Peptides in Drosophila

Expression of multiple reporter or effector transgenes in the same cell from a single construct is increasingly necessary in various experimental paradigms. The discovery of short, virus-derived peptide sequences that mediate a ribosome-skipping event enables generation of multiple separate peptide...

Descripción completa

Detalles Bibliográficos
Autores principales:	Daniels, Richard W., Rossano, Adam J., Macleod, Gregory T., Ganetzky, Barry
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4063965/ https://www.ncbi.nlm.nih.gov/pubmed/24945148 http://dx.doi.org/10.1371/journal.pone.0100637

Ejemplares similares

Autophagy promotes synapse development in Drosophila
por: Shen, Wei, et al.
Publicado: (2009)

A neuropeptide signaling pathway regulates synaptic growth in Drosophila
por: Chen, Xu, et al.
Publicado: (2012)

Loss of the Antimicrobial Peptide Metchnikowin Protects Against Traumatic Brain Injury Outcomes in Drosophila melanogaster
por: Swanson, Laura C., et al.
Publicado: (2020)

Split-QF System for Fine-Tuned Transgene Expression in Drosophila
por: Riabinina, Olena, et al.
Publicado: (2019)

A Drosophila model to investigate the neurotoxic side effects of radiation exposure
por: Sudmeier, Lisa J., et al.
Publicado: (2015)

Use of the viral 2A peptide for bicistronic expression in transgenic mice
por: Trichas, Georgios, et al.
Publicado: (2008)

Retrograde neurotrophin signaling through Tollo regulates synaptic growth in Drosophila
por: Ballard, Shannon L., et al.
Publicado: (2014)

A Novel Mutation in Brain Tumor Causes Both Neural Over-Proliferation and Neurodegeneration in Adult Drosophila
por: Loewen, Carin, et al.
Publicado: (2018)

Age and Diet Affect Genetically Separable Secondary Injuries that Cause Acute Mortality Following Traumatic Brain Injury in Drosophila
por: Katzenberger, Rebeccah J., et al.
Publicado: (2016)

Effects of blunt force injuries in third-instar Drosophila larvae persist through metamorphosis and reduce adult lifespan
por: Lika, Jorgo, et al.
Publicado: (2021)

Persistent Activation of the Innate Immune Response in Adult Drosophila Following Radiation Exposure During Larval Development
por: Sudmeier, Lisa J., et al.
Publicado: (2015)

A Drosophila Toolkit for the Visualization and Quantification of Viral Replication Launched from Transgenic Genomes
por: Wernet, Mathias F., et al.
Publicado: (2014)

Genetic variability affects absolute and relative potencies and kinetics of the anesthetics isoflurane and sevoflurane in Drosophila melanogaster
por: Olufs, Zachariah P. G., et al.
Publicado: (2018)

Beta-blockers reduce intestinal permeability and early mortality following traumatic brain injury in Drosophila
por: Scharenbrock, Amanda R, et al.
Publicado: (2021)

An efficient and multiple target transgenic RNAi technique with low toxicity in Drosophila
por: Qiao, Huan-Huan, et al.
Publicado: (2018)

Functional Expression of Drosophila para Sodium Channels : Modulation by the Membrane Protein TipE and Toxin Pharmacology
por: Warmke, Jeffrey W., et al.
Publicado: (1997)

The Making of Transgenic Drosophila guttifera
por: Shittu, Mujeeb, et al.
Publicado: (2020)

Transgenic tools for targeted chromosome rearrangements allow construction of balancer chromosomes in non-melanogaster Drosophila species
por: Stern, David L
Publicado: (2022)

Impact of age and vector construct on striatal and nigral transgene expression
por: Polinski, Nicole K, et al.
Publicado: (2016)

Ketogenic diet reduces early mortality following traumatic brain injury in Drosophila via the PPARγ ortholog Eip75B
por: Blommer, Joseph, et al.
Publicado: (2021)

Transgenic Tarantula Toxin: A novel tool to study mechanosensitive ion channels in Drosophila
por: Beqja, Denis, et al.
Publicado: (2020)

A polycistronic transgene design for combinatorial genetic perturbations from a single transcript in Drosophila
por: Teague, Alexander G., et al.
Publicado: (2023)

Retinal tissue preparation for high-resolution live imaging of photoreceptors expressing multiple transgenes
por: Haeri, Mohammad, et al.
Publicado: (2018)

An autoimmune disease with multiple molecular targets abrogated by the transgenic expression of a single autoantigen in the thymus
Publicado: (1993)

Strategies for Designing Transgenic DNA Constructs
por: Liu, Chengyu
Publicado: (2013)

Design of Gene Constructs for Transgenic Maize
por: Liu, Dong
Publicado: (2009)

Simultaneous tracking of movement and gene expression in multiple Drosophila melanogaster flies using GFP and DsRED fluorescent reporter transgenes
por: Grover, Dhruv, et al.
Publicado: (2009)

The Centenary of GENETICS: Bridges to the Future
por: Ganetzky, Barry, et al.
Publicado: (2016)

Transgenic Rice Expressing Amyloid β-peptide for Oral Immunization
por: Yoshida, Taiji, et al.
Publicado: (2011)

Amyloid peptides ABri and ADan show differential neurotoxicity in transgenic Drosophila models of familial British and Danish dementia
por: Marcora, María S, et al.
Publicado: (2014)

In Vivo RNAi Rescue in Drosophila melanogaster with Genomic Transgenes from Drosophila pseudoobscura
por: Langer, Christoph C. H., et al.
Publicado: (2010)

The production of viral vectors designed to express large and difficult to express transgenes within neurons
por: Holehonnur, Roopashri, et al.
Publicado: (2015)

Death following traumatic brain injury in Drosophila is associated with intestinal barrier dysfunction
por: Katzenberger, Rebeccah J, et al.
Publicado: (2015)

Phage integrases for the construction and manipulation of transgenic mammals
por: Hollis, Roger P, et al.
Publicado: (2003)

Transgene Expression and Host Cell Responses to Replication-Defective, Single-Cycle, and Replication-Competent Adenovirus Vectors
por: Crosby, Catherine M., et al.
Publicado: (2017)

Mammalian prion propagation in PrP transgenic Drosophila
por: Thackray, Alana M, et al.
Publicado: (2018)

Effect of kaempferol on the transgenic Drosophila model of Parkinson’s disease
por: Rahul, et al.
Publicado: (2020)

Effective CRISPR interference of an endogenous gene via a single transgene in mice
por: MacLeod, Ryan S., et al.
Publicado: (2019)

Dual Transgene Expression in Murine Cerebellar Purkinje Neurons by Viral Transduction In Vivo
por: Bosch, Marie K., et al.
Publicado: (2014)

Riboswitches for Controlled Expression of Therapeutic Transgenes Delivered by Adeno-Associated Viral Vectors
por: Tickner, Zachary J., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_sdeg3am0meoq0ri29vfid65n6d