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Tyrosinase-catalyzed site-specific immobilization of engineered C-phycocyanin to surface

Enzymatic crosslinking of proteins is often limited by the steric availability of the target residues, as of tyrosyl side chains in the case of tyrosinase. Carrying an N-terminal peptide-tag containing two tyrosine residues, the fluorescent protein C-phycocyanin HisCPC from Synechocystis sp. PCC6803...

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Detalles Bibliográficos
Autores principales: Faccio, Greta, Kämpf, Michael M., Piatti, Chiara, Thöny-Meyer, Linda, Richter, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064347/
https://www.ncbi.nlm.nih.gov/pubmed/24947668
http://dx.doi.org/10.1038/srep05370