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Tyrosinase-catalyzed site-specific immobilization of engineered C-phycocyanin to surface
Enzymatic crosslinking of proteins is often limited by the steric availability of the target residues, as of tyrosyl side chains in the case of tyrosinase. Carrying an N-terminal peptide-tag containing two tyrosine residues, the fluorescent protein C-phycocyanin HisCPC from Synechocystis sp. PCC6803...
Autores principales: | Faccio, Greta, Kämpf, Michael M., Piatti, Chiara, Thöny-Meyer, Linda, Richter, Michael |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4064347/ https://www.ncbi.nlm.nih.gov/pubmed/24947668 http://dx.doi.org/10.1038/srep05370 |
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