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Site-specific genomic (SSG) and random domain-localized (RDL) mutagenesis in yeast

BACKGROUND: A valuable weapon in the arsenal available to yeast geneticists is the ability to introduce specific mutations into yeast genome. In particular, methods have been developed to introduce deletions into the yeast genome using PCR fragments. These methods are highly efficient because they d...

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Detalles Bibliográficos
Autores principales: Gray, Misa, Kupiec, Martin, Honigberg, Saul M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC406509/
https://www.ncbi.nlm.nih.gov/pubmed/15090068
http://dx.doi.org/10.1186/1472-6750-4-7