Cargando…

Rapid isolation of yeast genomic DNA: Bust n' Grab

BACKGROUND: Mutagenesis of yeast artificial chromosomes (YACs) often requires analysis of large numbers of yeast clones to obtain correctly targeted mutants. Conventional ways to isolate yeast genomic DNA utilize either glass beads or enzymatic digestion to disrupt yeast cell wall. Using small glass...

Descripción completa

Detalles Bibliográficos
Autores principales: Harju, Susanna, Fedosyuk, Halyna, Peterson, Kenneth R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC406510/
https://www.ncbi.nlm.nih.gov/pubmed/15102338
http://dx.doi.org/10.1186/1472-6750-4-8