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Rapid isolation of yeast genomic DNA: Bust n' Grab
BACKGROUND: Mutagenesis of yeast artificial chromosomes (YACs) often requires analysis of large numbers of yeast clones to obtain correctly targeted mutants. Conventional ways to isolate yeast genomic DNA utilize either glass beads or enzymatic digestion to disrupt yeast cell wall. Using small glass...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2004
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC406510/ https://www.ncbi.nlm.nih.gov/pubmed/15102338 http://dx.doi.org/10.1186/1472-6750-4-8 |