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Three-dimensional super-resolution and localization of dense clusters of single molecules
When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to overlap, existing solutions to determine the number of molecules present and their precise...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066253/ https://www.ncbi.nlm.nih.gov/pubmed/24953078 http://dx.doi.org/10.1038/srep05388 |
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author | Barsic, Anthony Grover, Ginni Piestun, Rafael |
author_facet | Barsic, Anthony Grover, Ginni Piestun, Rafael |
author_sort | Barsic, Anthony |
collection | PubMed |
description | When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to overlap, existing solutions to determine the number of molecules present and their precise three-dimensional locations can tolerate little to no overlap. We propose a localization scheme that can identify several overlapping molecule images while maintaining high localization precision. A solution to this problem involving matched optical and digital techniques, as here proposed, can substantially increase the allowable labeling density and accelerate the data collection time of single-molecule localization microscopy by more than one order of magnitude. |
format | Online Article Text |
id | pubmed-4066253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-40662532014-06-23 Three-dimensional super-resolution and localization of dense clusters of single molecules Barsic, Anthony Grover, Ginni Piestun, Rafael Sci Rep Article When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to overlap, existing solutions to determine the number of molecules present and their precise three-dimensional locations can tolerate little to no overlap. We propose a localization scheme that can identify several overlapping molecule images while maintaining high localization precision. A solution to this problem involving matched optical and digital techniques, as here proposed, can substantially increase the allowable labeling density and accelerate the data collection time of single-molecule localization microscopy by more than one order of magnitude. Nature Publishing Group 2014-06-23 /pmc/articles/PMC4066253/ /pubmed/24953078 http://dx.doi.org/10.1038/srep05388 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | Article Barsic, Anthony Grover, Ginni Piestun, Rafael Three-dimensional super-resolution and localization of dense clusters of single molecules |
title | Three-dimensional super-resolution and localization of dense clusters of single molecules |
title_full | Three-dimensional super-resolution and localization of dense clusters of single molecules |
title_fullStr | Three-dimensional super-resolution and localization of dense clusters of single molecules |
title_full_unstemmed | Three-dimensional super-resolution and localization of dense clusters of single molecules |
title_short | Three-dimensional super-resolution and localization of dense clusters of single molecules |
title_sort | three-dimensional super-resolution and localization of dense clusters of single molecules |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066253/ https://www.ncbi.nlm.nih.gov/pubmed/24953078 http://dx.doi.org/10.1038/srep05388 |
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