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Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters
We describe a ribonucleic acid (RNA) reporter system for live-cell imaging of gene expression to detect changes in polymerase II activity on individual promoters in individual cells. The reporters use strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags) that ca...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066748/ https://www.ncbi.nlm.nih.gov/pubmed/24753407 http://dx.doi.org/10.1093/nar/gku297 |