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Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters

We describe a ribonucleic acid (RNA) reporter system for live-cell imaging of gene expression to detect changes in polymerase II activity on individual promoters in individual cells. The reporters use strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags) that ca...

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Detalles Bibliográficos
Autores principales:	Shin, Ilchung, Ray, Judhajeet, Gupta, Vinayak, Ilgu, Muslum, Beasley, Jonathan, Bendickson, Lee, Mehanovic, Samir, Kraus, George A., Nilsen-Hamilton, Marit
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4066748/ https://www.ncbi.nlm.nih.gov/pubmed/24753407 http://dx.doi.org/10.1093/nar/gku297

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