Improved elongation factor-1 alpha-based vectors for stable high-level expression of heterologous proteins in Chinese hamster ovary cells

BACKGROUND: Establishing highly productive clonal cell lines with constant productivity over 2–3 months of continuous culture remains a tedious task requiring the screening of tens of thousands of clonal colonies. In addition, long-term cultivation of many candidate lines derived in the absence of d...

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Detalles Bibliográficos
Autores principales: Orlova, Nadezhda A, Kovnir, Sergey V, Hodak, Julia A, Vorobiev, Ivan I, Gabibov, Alexandre G, Skryabin, Konstantin G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067061/
https://www.ncbi.nlm.nih.gov/pubmed/24929670
http://dx.doi.org/10.1186/1472-6750-14-56

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067061/
https://www.ncbi.nlm.nih.gov/pubmed/24929670
http://dx.doi.org/10.1186/1472-6750-14-56

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