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Improved elongation factor-1 alpha-based vectors for stable high-level expression of heterologous proteins in Chinese hamster ovary cells

BACKGROUND: Establishing highly productive clonal cell lines with constant productivity over 2–3 months of continuous culture remains a tedious task requiring the screening of tens of thousands of clonal colonies. In addition, long-term cultivation of many candidate lines derived in the absence of d...

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Detalles Bibliográficos
Autores principales:	Orlova, Nadezhda A, Kovnir, Sergey V, Hodak, Julia A, Vorobiev, Ivan I, Gabibov, Alexandre G, Skryabin, Konstantin G
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067061/ https://www.ncbi.nlm.nih.gov/pubmed/24929670 http://dx.doi.org/10.1186/1472-6750-14-56

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