A Modified Janus Cassette (Sweet Janus) to Improve Allelic Replacement Efficiency by High-Stringency Negative Selection in Streptococcus pneumoniae

The Janus cassette permits marker-free allelic replacement or knockout in streptomycin-resistant Streptococcus pneumoniae (pneumococcus) through sequential positive and negative selection. Spontaneous revertants of Janus can lead to high level of false-positives during negative selection, which nece...

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Detalles Bibliográficos
Autores principales: Li, Yuan, Thompson, Claudette M., Lipsitch, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068995/
https://www.ncbi.nlm.nih.gov/pubmed/24959661
http://dx.doi.org/10.1371/journal.pone.0100510

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068995/
https://www.ncbi.nlm.nih.gov/pubmed/24959661
http://dx.doi.org/10.1371/journal.pone.0100510

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