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Chimeric Avidin – NMR Structure and Dynamics of a 56 kDa Homotetrameric Thermostable Protein

Chimeric avidin (ChiAVD) is a product of rational protein engineering remarkably resistant to heat and harsh conditions. In quest of the fundamentals behind factors affecting stability we have elucidated the solution NMR spectroscopic structure of the biotin–bound form of ChiAVD and characterized th...

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Detalles Bibliográficos
Autores principales: Tossavainen, Helena, Kukkurainen, Sampo, Määttä, Juha A. E., Kähkönen, Niklas, Pihlajamaa, Tero, Hytönen, Vesa P., Kulomaa, Markku S., Permi, Perttu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4069078/
https://www.ncbi.nlm.nih.gov/pubmed/24959850
http://dx.doi.org/10.1371/journal.pone.0100564