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A broad survey reveals substitution tolerance of residues ligating FeS clusters in [NiFe] hydrogenase

BACKGROUND: In order to understand the effects of FeS cluster attachment in [NiFe] hydrogenase, we undertook a study to substitute all 12 amino acid positions normally ligating the three FeS clusters in the hydrogenase small subunit. Using the hydrogenase from Alteromonas macleodii “deep ecotype” as...

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Detalles Bibliográficos
Autores principales: Yonemoto, Isaac T, Clarkson, Benjamin R, Smith, Hamilton O, Weyman, Philip D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070099/
https://www.ncbi.nlm.nih.gov/pubmed/24934472
http://dx.doi.org/10.1186/1471-2091-15-10