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Comparison of RNA-Seq by poly (A) capture, ribosomal RNA depletion, and DNA microarray for expression profiling

BACKGROUND: RNA sequencing (RNA-Seq) is often used for transcriptome profiling as well as the identification of novel transcripts and alternative splicing events. Typically, RNA-Seq libraries are prepared from total RNA using poly(A) enrichment of the mRNA (mRNA-Seq) to remove ribosomal RNA (rRNA),...

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Detalles Bibliográficos
Autores principales: Zhao, Wei, He, Xiaping, Hoadley, Katherine A, Parker, Joel S, Hayes, David Neil, Perou, Charles M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070569/
https://www.ncbi.nlm.nih.gov/pubmed/24888378
http://dx.doi.org/10.1186/1471-2164-15-419