Effects of the Anaerobic Respiration of Shewanella oneidensis MR-1 on the Stability of Extracellular U(VI) Nanofibers

Uranium (VI) is considered to be one of the most widely dispersed and problematic environmental contaminants, due in large part to its high solubility and great mobility in natural aquatic systems. We previously reported that under anaerobic conditions, Shewanella oneidensis MR-1 grown in medium con...

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Autores principales: Jiang, Shenghua, Hur, Hor-Gil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070970/
https://www.ncbi.nlm.nih.gov/pubmed/23719584
http://dx.doi.org/10.1264/jsme2.ME12149
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author Jiang, Shenghua
Hur, Hor-Gil
author_facet Jiang, Shenghua
Hur, Hor-Gil
author_sort Jiang, Shenghua
collection PubMed
description Uranium (VI) is considered to be one of the most widely dispersed and problematic environmental contaminants, due in large part to its high solubility and great mobility in natural aquatic systems. We previously reported that under anaerobic conditions, Shewanella oneidensis MR-1 grown in medium containing uranyl acetate rapidly accumulated long, extracellular, ultrafine U(VI) nanofibers composed of polycrystalline chains of discrete meta-schoepite (UO(3)·2H(2)O) nanocrystallites. Wild-type MR-1 finally transformed the uranium (VI) nanofibers to uranium (IV) nanoparticles via further reduction. In order to investigate the influence of the respiratory chain in the uranium transformation process, a series of mutant strains lacking a periplasmic cytochrome MtrA, outer membrane (OM) cytochrome MtrC and OmcA, a tetraheme cytochrome CymA anchored to the cytoplasmic membrane, and a trans-OM protein MtrB, were tested in this study. Although all the mutants produced U(VI) nanofibers like the wild type, the transformation rates from U(VI) nanofibers to U(IV) nanoparticles varied; in particular, the mutant with deletion in tetraheme cytochrome CymA stably maintained the uranium (VI) nanofibers, suggesting that the respiratory chain of S. oneidensis MR-1 is probably involved in the stability of extracellular U(VI) nanofibers, which might be easily treated via the physical processes of filtration or flocculation for the remediation of uranium contamination in sediments and aquifers, as well as the recovery of uranium in manufacturing processes.
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spelling pubmed-40709702014-07-24 Effects of the Anaerobic Respiration of Shewanella oneidensis MR-1 on the Stability of Extracellular U(VI) Nanofibers Jiang, Shenghua Hur, Hor-Gil Microbes Environ Articles Uranium (VI) is considered to be one of the most widely dispersed and problematic environmental contaminants, due in large part to its high solubility and great mobility in natural aquatic systems. We previously reported that under anaerobic conditions, Shewanella oneidensis MR-1 grown in medium containing uranyl acetate rapidly accumulated long, extracellular, ultrafine U(VI) nanofibers composed of polycrystalline chains of discrete meta-schoepite (UO(3)·2H(2)O) nanocrystallites. Wild-type MR-1 finally transformed the uranium (VI) nanofibers to uranium (IV) nanoparticles via further reduction. In order to investigate the influence of the respiratory chain in the uranium transformation process, a series of mutant strains lacking a periplasmic cytochrome MtrA, outer membrane (OM) cytochrome MtrC and OmcA, a tetraheme cytochrome CymA anchored to the cytoplasmic membrane, and a trans-OM protein MtrB, were tested in this study. Although all the mutants produced U(VI) nanofibers like the wild type, the transformation rates from U(VI) nanofibers to U(IV) nanoparticles varied; in particular, the mutant with deletion in tetraheme cytochrome CymA stably maintained the uranium (VI) nanofibers, suggesting that the respiratory chain of S. oneidensis MR-1 is probably involved in the stability of extracellular U(VI) nanofibers, which might be easily treated via the physical processes of filtration or flocculation for the remediation of uranium contamination in sediments and aquifers, as well as the recovery of uranium in manufacturing processes. Japanese Society of Microbial Ecology/The Japanese Society of Soil Microbiology 2013-09 2013-05-29 /pmc/articles/PMC4070970/ /pubmed/23719584 http://dx.doi.org/10.1264/jsme2.ME12149 Text en Copyright © 2013 by the Japanese Society of Microbial Ecology / the Japanese Society of Soil Microbiology http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Jiang, Shenghua
Hur, Hor-Gil
Effects of the Anaerobic Respiration of Shewanella oneidensis MR-1 on the Stability of Extracellular U(VI) Nanofibers
title Effects of the Anaerobic Respiration of Shewanella oneidensis MR-1 on the Stability of Extracellular U(VI) Nanofibers
title_full Effects of the Anaerobic Respiration of Shewanella oneidensis MR-1 on the Stability of Extracellular U(VI) Nanofibers
title_fullStr Effects of the Anaerobic Respiration of Shewanella oneidensis MR-1 on the Stability of Extracellular U(VI) Nanofibers
title_full_unstemmed Effects of the Anaerobic Respiration of Shewanella oneidensis MR-1 on the Stability of Extracellular U(VI) Nanofibers
title_short Effects of the Anaerobic Respiration of Shewanella oneidensis MR-1 on the Stability of Extracellular U(VI) Nanofibers
title_sort effects of the anaerobic respiration of shewanella oneidensis mr-1 on the stability of extracellular u(vi) nanofibers
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4070970/
https://www.ncbi.nlm.nih.gov/pubmed/23719584
http://dx.doi.org/10.1264/jsme2.ME12149
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