Galactose Oxidase from Fusarium oxysporum - Expression in E. coli and P. pastoris and Biochemical Characterization

A gene coding for galactose 6-oxidase from Fusarium oxysporum G12 was cloned together with its native preprosequence and a C-terminal His-tag, and successfully expressed both in Escherichia coli and Pichia pastoris. The enzyme was subsequently purified and characterized. Among all tested substrates,...

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Detalles Bibliográficos
Autores principales: Paukner, Regina, Staudigl, Petra, Choosri, Withu, Sygmund, Christoph, Halada, Petr, Haltrich, Dietmar, Leitner, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072685/
https://www.ncbi.nlm.nih.gov/pubmed/24967652
http://dx.doi.org/10.1371/journal.pone.0100116

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4072685/
https://www.ncbi.nlm.nih.gov/pubmed/24967652
http://dx.doi.org/10.1371/journal.pone.0100116

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