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A Chemical Strategy for the Cell-Based Detection of HDAC Activity
[Image: see text] A strategy for activity-based enzyme detection using a novel enamide-based chemical strategy is described. Enzymatic cleavage of an amide bond results in the formation of an aldehyde. The interaction of this aldehyde with proteins increases retention in cells that express the enzym...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical
Society
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4076023/ https://www.ncbi.nlm.nih.gov/pubmed/25056147 http://dx.doi.org/10.1021/cb500248r |
| _version_ | 1782323437757267968 |
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| author | Diyabalanage, Himashinie V. K. Van de Bittner, Genevieve C. Ricq, Emily L. Hooker, Jacob M. |
| author_facet | Diyabalanage, Himashinie V. K. Van de Bittner, Genevieve C. Ricq, Emily L. Hooker, Jacob M. |
| author_sort | Diyabalanage, Himashinie V. K. |
| collection | PubMed |
| description | [Image: see text] A strategy for activity-based enzyme detection using a novel enamide-based chemical strategy is described. Enzymatic cleavage of an amide bond results in the formation of an aldehyde. The interaction of this aldehyde with proteins increases retention in cells that express the enzyme. Proof of concept for this enamide-based strategy is demonstrated by detecting histone deacetylase (HDAC) activity in HeLa cells. The modular design of this strategy makes it amenable to in vitro and in vivo detection. |
| format | Online Article Text |
| id | pubmed-4076023 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2014 |
| publisher | American Chemical
Society |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-40760232015-04-28 A Chemical Strategy for the Cell-Based Detection of HDAC Activity Diyabalanage, Himashinie V. K. Van de Bittner, Genevieve C. Ricq, Emily L. Hooker, Jacob M. ACS Chem Biol [Image: see text] A strategy for activity-based enzyme detection using a novel enamide-based chemical strategy is described. Enzymatic cleavage of an amide bond results in the formation of an aldehyde. The interaction of this aldehyde with proteins increases retention in cells that express the enzyme. Proof of concept for this enamide-based strategy is demonstrated by detecting histone deacetylase (HDAC) activity in HeLa cells. The modular design of this strategy makes it amenable to in vitro and in vivo detection. American Chemical Society 2014-04-28 2014-06-20 /pmc/articles/PMC4076023/ /pubmed/25056147 http://dx.doi.org/10.1021/cb500248r Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) |
| spellingShingle | Diyabalanage, Himashinie V. K. Van de Bittner, Genevieve C. Ricq, Emily L. Hooker, Jacob M. A Chemical Strategy for the Cell-Based Detection of HDAC Activity |
| title | A Chemical Strategy for the Cell-Based Detection of
HDAC Activity |
| title_full | A Chemical Strategy for the Cell-Based Detection of
HDAC Activity |
| title_fullStr | A Chemical Strategy for the Cell-Based Detection of
HDAC Activity |
| title_full_unstemmed | A Chemical Strategy for the Cell-Based Detection of
HDAC Activity |
| title_short | A Chemical Strategy for the Cell-Based Detection of
HDAC Activity |
| title_sort | chemical strategy for the cell-based detection of
hdac activity |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4076023/ https://www.ncbi.nlm.nih.gov/pubmed/25056147 http://dx.doi.org/10.1021/cb500248r |
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