Mixed Disulfide Formation at Cys141 Leads to Apparent Unidirectional Attenuation of Aspergillus niger NADP-Glutamate Dehydrogenase Activity

NADP-Glutamate dehydrogenase from Aspergillus niger (AnGDH) exhibits sigmoid 2-oxoglutarate saturation. Incubation with 2-hydroxyethyl disulfide (2-HED, the disulfide of 2-mercaptoethanol) resulted in preferential attenuation of AnGDH reductive amination (forward) activity but with a negligible effe...

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Detalles Bibliográficos
Autores principales: Walvekar, Adhish S., Choudhury, Rajarshi, Punekar, Narayan S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079599/
https://www.ncbi.nlm.nih.gov/pubmed/24987966
http://dx.doi.org/10.1371/journal.pone.0101662

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079599/
https://www.ncbi.nlm.nih.gov/pubmed/24987966
http://dx.doi.org/10.1371/journal.pone.0101662

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