Theoretical and experimental assessment of degenerate primer tagging in ultra-deep applications of next-generation sequencing

Primer IDs (pIDs) are random oligonucleotide tags used in next-generation sequencing to identify sequences that originate from the same template. These tags are produced by degenerate primers during the reverse transcription of RNA molecules into cDNA. The use of pIDs helps to track the number of RN...

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Detalles Bibliográficos
Autores principales: Liang, Richard H., Mo, Theresa, Dong, Winnie, Lee, Guinevere Q., Swenson, Luke C., McCloskey, Rosemary M., Woods, Conan K., Brumme, Chanson J., Ho, Cynthia K.Y., Schinkel, Janke, Joy, Jeffrey B., Harrigan, P. Richard, Poon, Art F.Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081055/
https://www.ncbi.nlm.nih.gov/pubmed/24810852
http://dx.doi.org/10.1093/nar/gku355

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081055/
https://www.ncbi.nlm.nih.gov/pubmed/24810852
http://dx.doi.org/10.1093/nar/gku355

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