Validation of a fluorescence-based screening concept to identify ribosome assembly defects in Escherichia coli

While the structure of mature ribosomes is analyzed in atomic detail considerably less is known about their assembly process in living cells. This is mainly due to technical and conceptual hurdles. To analyze ribosome assembly in vivo, we designed and engineered an Escherichiacoli strain—using chrom...

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Detalles Bibliográficos
Autores principales: Nikolay, Rainer, Schloemer, Renate, Schmidt, Sabine, Mueller, Silke, Heubach, Anja, Deuerling, Elke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081057/
https://www.ncbi.nlm.nih.gov/pubmed/24792169
http://dx.doi.org/10.1093/nar/gku381

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081057/
https://www.ncbi.nlm.nih.gov/pubmed/24792169
http://dx.doi.org/10.1093/nar/gku381

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