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Validation of a fluorescence-based screening concept to identify ribosome assembly defects in Escherichia coli
While the structure of mature ribosomes is analyzed in atomic detail considerably less is known about their assembly process in living cells. This is mainly due to technical and conceptual hurdles. To analyze ribosome assembly in vivo, we designed and engineered an Escherichiacoli strain—using chrom...
Autores principales: | Nikolay, Rainer, Schloemer, Renate, Schmidt, Sabine, Mueller, Silke, Heubach, Anja, Deuerling, Elke |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4081057/ https://www.ncbi.nlm.nih.gov/pubmed/24792169 http://dx.doi.org/10.1093/nar/gku381 |
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